Nahid Ghaed Amini; Mohammad Fazilati; Saeid Habib-Allahi; Habib-Allah Nazem; Seyed Hossein Hejazi
Abstract
Despite the many chemical drugs available, the prevalence and mortality rate of melanoma is high. Therefore, the use of drugs of natural origin with lower cost and higher efficiency can be a good way to save patients. Therefore, in this study, the main purpose of this study was to evaluate the effects ...
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Despite the many chemical drugs available, the prevalence and mortality rate of melanoma is high. Therefore, the use of drugs of natural origin with lower cost and higher efficiency can be a good way to save patients. Therefore, in this study, the main purpose of this study was to evaluate the effects of Moringa extract on melanoma. Hydroalcoholic extract of Moringa leaves was prepared. After examining the total phenolic and flavonoid content of the extract, their type was confirmed by HPLC and spectrophotometer methods. The antioxidant power was measured by DPPH free radical scavenging and FRPA methods. Then the tumor was induced in C57BL6 mice and then the effects of the extract were evaluated on B16F10 cell line and tumor mice. The total flavonoid content was 60.65±1.75 mg quercetin acid per gram of dry extract and the total phenol content was 20.25±1.23 mg gallic acid per gram of dry extract. Three phenolic and flavonoid compounds with antioxidant properties identified include quercetin, gallic acid, and caffeic acid. The free radical scavenging power and the reducing power of the extract increased with increasing the concentration. The IC50 of moringa extract was 73 μM/mol. The tumor volume was significantly reduced by different doses of the extract in two weeks. The results of present study show the positive effect of Moringa leaves extract in reducing the survival rate of B16F10 melanoma cancer cells and tumor volume in mice.
